Skip to main content
. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Cell Rep. 2018 Oct 9;25(2):357–367.e4. doi: 10.1016/j.celrep.2018.09.039

Figure 2. Treatment Initiated at 6 Weeks Rescues PC Survival and Impaired Spontaneous Firing and Excitability in PC Tsc1 Mice.

Figure 2.

Recordings were performed at 11 weeks of age (5 weeks after treatment initiation at 6 weeks of age).

(A) PC loss is rescued with rapamycin therapy (quantification of PCs on left). Shown are midline vermis sagittal sections stained with calbindin (to identify PCs) and phosphoS6 (to evaluate mTOR activity) antibodies.

(B and C) Rapamycin initiated at 6 weeks rescues (B) intrinsic firing properties and (C) excitability deficits in mutant mice.

Two-way ANOVA, Bonferroni post hoc analysis. *p < 0.05, **p < 0.01, and ***p < 0.001. Ns, not significant; Rapa, rapamycin; VEH, vehicle. Data are reported as mean ± SEM.