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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Endocrinol. 2018 Dec 1;239(3):289–301. doi: 10.1530/JOE-18-0282

Figure 1. GH Acutely Induces Lipolysis and Reduces FSP27 Expression in a Time and Dose Dependent Manner.

Figure 1.

a) qPCR analysis of mRNA levels of regulators of lipolysis: ATGL, HSL, Perilipin, Cidea, Comparative gene identification-58 (CGI-58), Tail-interacting protein of 47 kD (TIP47), G0/G1 switch gene 2 (G0S2), and FSP27 mRNA was compared in RNA isolated from perigonadal fat of 4 month old male bGH mice. Data are shown as mean ± SEM of 8–10 Samples. * p<0.05; **p<0.01; ***p<0.01.

b) qPCR analysis of ATGL, HSL, Perilipin, Cidea, CGI-58, TIP47, and FSP27 mRNA in RNA isolated from 3T3-L1 adipocytes treated with 500ng/mL recombinant bovine GH (bGH) for 2 hours. Data are shown as mean ± SEM of 3 independent experiments.

c) qPCR and Western Blot analysis of FSP27 mRNA and protein isolated from 3T3-L1 adipocytes treated with 500ng/mL recombinant bovine GH (bGH). Data are shown as mean ± SEM of 3 independent experiments.

d) Lipolysis as measured by glycerol release from 3T3-L1 adipocytes treated with bGH for two hours. Data are shown as mean ± SEM of 3 independent experiments.

e) qPCR and Western Blot analysis of FSP27 mRNA and protein isolated from 3T3-L1 adipocytes treated with bGH for 2 hours. Data are shown as mean ± SEM of 3 independent experiments.