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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Endocrinol. 2018 Dec 1;239(3):289–301. doi: 10.1530/JOE-18-0282

Figure 5. FSP27 Over-expression Prevents GH-Induced Lipolysis and Insulin Resistance.

Figure 5.

a) Representative Western blot analysis of FSP27 in WT 3T3-L1 adipocytes and FSP27 over-expressing adipocytes treated with 500ng/mL bGH for two hours.

b) Lipolysis as measured by glycerol release from WT 3T3-L1 adipocytes and FSP27 over-expressing adipocytes treated with 250ng/mL bGH for two hours. Data are shown as mean ± SEM of 3 independent experiments. Asterisks indicate a significant differences * p<0.05, ** p<0.01, *** p<0.001

c) Immunofluorescence of Nile Red Stained WT and FSP27 over-expressing 3T3-L1 adipocytes treated with vehicle or 500ng/mL bGH for 24 hours.

d) Representative Western blot analysis of pAKT S473 and total AKT in WT 3T3-L1 adipocytes and FSP27 over-expressing adipocytes treated with 250ng/mL bGH for two hours and 10nM insulin for 15 minutes.

d) Proposed mechanism of GH-induced lipolysis.