Fig 5. In vitro BMDC migration is reduced by developmental AHR activation.
(A) Bone marrow dendritic cells (BMDC) were generated from bone marrow cells of uninfected offspring of vehicle and TCDD treated dams. (B-D) The bar graphs depict the number of bone marrow cells (B), the number of immature BMDC (C), and the number of mature CD11chiMHCIIhi BMDCs (D) from adult offspring in each group. (E, F) Migration towards a gradient of CCL21 was determined using a Transwell system. (E) The graph depicts the average number of BMDCs collected from the bottom well at each CCL21 concentration. p-values are listed above data points. (F) The bar graph shows the average area under the curve (AUC) for DC migration in the two treatment groups. Error bars depict ± SEM. An * indicates p ≤ 0.05. BMDCs from all offspring within a group are from a separate dam (n = 12 mice per group). Data were combined from two independently conducted experiments. Underlying data can be found in S1 Data.