Fig 7. CCR7 levels on resident or recently migrated DC are not changed with early life exposure to TCDD in the MLN.

At maturity, developmentally exposed offspring were infected with H3N2 IAV (HKx31). CFSE (8mM) was instilled (i.n.) 54 h after infection. Mice were sacrificed 18 h after CFSE treatment and single cell suspensions were made from MLN. Vehicle (V, solid line), TCDD (T, dotted line). Grey histograms indicate the fluorescence minus one (FMO) control. (A-D) Representative histograms depict CCR7 levels on recently migrated (CFSE⁺) DC subsets in the MLN 3 days after IAV infection. (E-H) Representative histograms depict CCR7 levels three days after infection on resident CFSEˉ DC subsets in MLNs of vehicle or TCDD exposed offspring. Error bars depict ± SEM. All offspring within a group are from a separate dam (n = 3–9 mice per group per day). Data are representative of at least two experiments with similar results.