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. 2018 Oct 18;17(18):2309–2320. doi: 10.1080/15384101.2018.1535219

Figure 1.

Figure 1.

Spheroid cells had stem cell–like characteristics. (a) Images of the spheroids generated from adherent JEG-3 cells were shown from day 0 to day 6 cultured in serum-free media. Scale bar, 100 μm. (a) Day 0, (b) day 2, (c) day 4, and (d) day 6. (b) Clonogenicity of spheroids and adherent cells. Images of a single spheroid cell cultured in serum-free media were shown. (a) Day 1, (b) day 3, (c) day 5, (d) day 7, and (e) quantitation of colony-forming capabilities. (c) Stemness-related genes were examined using RT-PCR. (d) Drug resistance: spheroids and adherent cells were incubated with MTX (left) or VP16 (right), and the viability was measured using CCK-8 assay. (e) Flow cytometric analysis of the surface expression of CD44 and CD133. Each bar represents mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.