Figure 6.

AhR transcriptionally activated ABCG2. (a) AhR-binding sites in ABCG2 promoter were marked with a box. Luciferase reporter assay was performed in JEG-3 cells transfected with shAhR or treated with TCDD and controls. (b) ChIP analysis of AhR binding to the ABCG2 promotor in the JEG-3 cells. qPCR was performed with primer specific to regions around the AhR-binding motifs (P1) and a control region (P2). (c) RT-PCR analysis of the ABCG2 level in JEG-3 cells (left) and BeWo cells (right) with AhR knockdown or activation compared with controls. (d) Western blot analysis results of JEG-3 (left) and BeWo (right) cells with or without AhR activation were shown. (e) RT-PCR analysis of the mRNA expression of ABCG2 in spheroids and JEG-3 cells. (f) Expression of ABCG2 detected in two groups using Western blot analysis was shown. Each bar represents mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.