Figure 1.

MicroRNA-378 disrupted the mouse estrous cycle. (a) The expression of miR-378-3p and miR-378-5p determined by quantitative PCR. (b) Estrous cycle after ovary-subcutaneous injection. The mice were injected at dioestrum. They were at the same stage of estrous cycle over the next 2–3 d, then the estrus of mice with miR-378 overexpression lasted much longer than that of control group (4 d in miR-378 group and 2 d in GFP group). (c) Aromatase expression after microinjection of GFP control, miR-378, Inhibitors and miR-378+ inhibitors determined by western blotting. Data are Mean ± SD of at least 3 independent experiments; *P < 0.05; **P < 0.01; ***P < 0.001.