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. 2018 Oct 15;7:e40474. doi: 10.7554/eLife.40474

Figure 4. Activation of Ral triggers invasion of non-transformed cells.

(A) Transwell invasion assay with OptoControl and OptoRal cells, in the dark or under light stimulation. Graph shows the mean ±SEM from three independent experiments. In the graph the results of one-way ANOVA tests are reported to compare pairs of conditions. (B) Transwell invasion assay with OptoControl iRFP-Abi1 wild-type, OptoRal iRFP-Abi1 wild-type, and OptoRal iRFP-Abi1Q56A cells, in the dark or under light stimulation. Graph shows the mean ±SEM from five independent experiments. In the graph the results of one-way ANOVA tests are reported to compare pairs of conditions. *p<0.05, **p<0.01, ***, p<0.001, ns not-significant.

Figure 4.

Figure 4—figure supplement 1. The custom-made illumination device for optogenetic activation on multi-well dishes.

Figure 4—figure supplement 1.

(A) The device consists of an array of 12 blue LEDs mounted in a 3D-printed enclosure so that each LED illuminates one well only of a 12-well plate. Cells were illuminated from the bottom of the plate and the device was kept in a cell-culture incubator for the whole duration of the experiments. (B) Each LED is controlled independently through an Arduino Due micro-controller.