Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell Line (H. sapiens) |
HEK293T | American Type Culture Collection |
Cat# CRL-3216 RRID:CVCL_0063 |
|
| Recombinant DNA reagent |
pcDNA3.1-3xFLAG-TEV-IFRD2 | This paper | N/A | Mammalian expression vector expressing Flag-tagged IFRD2 behind a CMV promoter |
| Antibody | IFRD2 | Invitrogen | Cat# PA5-48833 RRID:AB_2634289 |
IB: 1:1000 |
| Antibody | FLAG M2 | Sigma-Aldrich | Cat# F3165 RRID:AB_259529 |
IB: 1:5000 |
| Antibody | uL2 | Abcam | Cat# ab169538 RRID:AB_2714187 |
IB: 1:10000 |
| Antibody | eS24 | Abcam | Cat# ab196652 RRID:AB)2714188 |
IB: 1:3000 |
| Antibody | HRP anti-mouse | Jackson ImmunoResearch | Cat# 115-035-003 RRID:AB_10015289 |
IB: 1:5000 |
| Antibody | HRP anti-rabbit | Jackson ImmunoResearch | Cat# 111-035-003 RRID:AB_2313567 |
IB: 1:5000 |
| Antibody | FLAG M2 agarose resin |
Sigma-Aldrich | Cat# A2220 RRID:AB_10063035 |
|
| Peptide, recombinant protein |
3X FLAG peptide | Sigma-Aldrich | Cat# F4799 | |
| Peptide, recombinant protein |
RNase A | Sigma-Aldrich | Cat# R6513 | |
| Chemical compound, drug |
Dulbecco's Modified Eagle Medium (DMEM) | Gibco/Thermo Fisher | Cat# 10569 | |
| Chemical compound, drug |
HyClone Fetal Bovine Serum |
GE Healthcare Life Sciences | Cat# SH30910.03 | |
| Chemical compound, drug |
Trypsin-EDTA | Gibco/Thermo Fisher | Cat# 25200 | |
| Chemical compound, drug |
OptiMEM | Gibco/Thermo Fisher | Cat# 31985 | |
| Chemical compound, drug |
TransIT 293 | Mirus | Cat# MIR2705 | |
| Chemical compound, drug |
EDTA | GrowCells | Cat# MRGF-1202 | |
| Chemical compound, drug |
SuperSignal West Pico |
Thermo Fisher | Cat# 34080 | |
| Biological sample (O. cuniculus) |
Rabbit reticulocyte lysate |
Green Hectares | N/A | |
| Software, algorithm |
RELION-2.0 or RELION-2.1 |
Kimanius et al., 2016 | RRID:SCR_016274 | https://www2.mrc-lmb.cam.ac.uk/relion |
| Software, algorithm |
MotionCor2 | Zheng et al., 2017 | RRID:SCR_016499 | http://msg.ucsf.edu/em/software/motioncor2.html |
| Software, algorithm |
GCTF | Zhang, 2016 | RRID:SCR_016500 | https://www.mrc-lmb.cam.ac.uk/kzhang/ |
| Software, algorithm |
UCSF Chimera | Pettersen et al., 2004 | RRID:SCR_004097 | https://www.cgl.ucsf.edu/chimera/ |
| Software, algorithm |
Coot v.0.8.9 | Brown et al., 2015a | RRID:SCR_014222 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Software, algorithm |
I-TASSER | Zhang, 2008 | RRID:SCR_014627 | https://zhanglab.ccmb.med.umich.edu/I-TASSER/ |
| Software, algorithm |
PHENIX | Adams et al., 2010 | RRID:SCR_014224 | https://www.phenix-online.org/ |
| Software, algorithm |
MolProbity v.4.3.1 | Chen et al., 2010 | RRID:SCR_014226 | http://molprobity.biochem.duke.edu/ |
| Software, algorithm |
EMRinger | Barad et al., 2015 | N/A | http://emringer.com/ |
| Software, algorithm |
SBGrid | Morin et al., 2013 | RRID:SCR_003511 | https://sbgrid.org/ |
| Software, algorithm |
PyMOL | DeLano, 2002 | RRID:SCR_000305 | http://www.pymol.org |
| Software, algorithm |
SCIPION | de la Rosa-Trevín et al., 2016 | N/A | http://scipion.i2pc.es/ |
| Software, algorithm |
MonoRes | Vilas et al., 2018 | N/A |