(A) Representative sensogram of the wildtype Yan SAM interaction, via SPR. Concentrations of analyte are color-coded and listed to the right, and the kinetic model is represented by dashed lines. (B) Sensogram of the SY5 interaction, demonstrating stronger binding affinity. (C) Table of parameters obtained from kinetic fitting of sensograms for Yan and SY1–5 interactions. Two technical replicates were taken for each interaction to calculate parameters. (D) Co-immunoprecipitation of Yan and SY1–5 constructs. IPs are shown in the top panel, whereas lysate inputs are shown in the bottom panel. FLAG-tagged Yan constructs were immunoprecipitated and both HA-tagged and FLAG-tagged proteins were immunoblotted. Arrows mark the mobility of the Yan constructs, whereas asterisks mark unreduced IgG in the IP and a non-specific FLAG-reactive species in the lysate, respectively. Fold enrichment of IP to lysate relative to wildtype is noted.