Figure 7. Salm is a direct Yan target and is specifically derepressed in yan^SY5 mutants.

(A–D) ChIP-seq tracks of Yan binding in third instar larval eye discs, represented as smoothed tag density with a scale of number of reads per million; alignment was performed to Drosophila genome assembly Dm3. For clarity, the longest isoform of each gene is depicted, with 5’ and 3’ ends noted. Arrows mark the location of the primers used for ChIP-qPCR; gray boxes mark the location of peaks called by MACS (see Figure 7—source data 1). (A) spalt major locus (salm). (B) prospero locus (pros). (C) yan locus (aop). (D) argos locus (aos). (E) ChIP-qPCR of wildtype and yan^SY5 eye discs. Error bars represent SEM of n = 3 biological replicates. Significance was calculated by Student’s T-test *, p<0.05. (F) Transcription assays with the spalt reporter. Error bars represent SEM of n = 4 biological replicates. Significance was calculated by Student’s T-test *, p<0.05; n.s., not significant. (G–H) spalt reporter-driven GFP expression, in a wildtype (G) and SY5 background (H). (I–J) pros reporter-driven GFP expression, in a wildtype (I) and SY5 background (J). Scale bars represent five microns.

Figure 7—figure supplement 1. Comparison of Yan ChIP patterns in the eye disc versus embryo reveals eye-specific occupancy at salm.

(A-D) ChIP-seq tracks of Yan binding in third instar larval eye discs, as in Figure 7, compared to embryo ChIP-seq (Webber et al., 2013). Tracks are represented as smoothed tag density with a scale of number of reads per million; alignment was performed to Drosophila genome assembly Dm5. For clarity, the longest isoform of each gene is depicted, with 5′ and 3′ ends noted. Arrows mark location of primer binding for ChIP-qPCR; gray boxes mark the location of peaks called by MACS (see Figure 7—source data 1). (A) spalt major locus (salm). (B) spalt-related locus (salr). (C) prospero locus (pros). (D) yan locus (aop). (E) argos locus (aos).