Figure 3.

Myeloid-Specific Deletion of SHIP-1 Improves Trained Immunity In Vivo

(A) In vivo model of training by two intraperitoneal (i.p.) β-glucan injections and secondary i.p. LPS challenge for measuring serum cytokines.

(B) Mice were treated according to Figure 3A. Serum was collected after 60 min (TNFα) or 90 min (IL-1β and IL-6) of LPS challenge, and cytokines were analyzed.

(C) In vivo model of training as in (A) but with secondary Candida albicans lethal infection.

(D) Survival curve according to Figure 3C.

(E) In vivo model of training by a systemic infection with a low dose of C. albicans followed by a secondary lethal challenge with the same pathogen.

(F) Survival curve according to Figure 3E.

(G and H) Renal cytokines on day 2 post-infection (p.i.) (G) and kidney fungal burden (H) at indicated time points p.i. were evaluated in trained mice, following model in Figure 3E.

In (B), (G), and (H), single dots correspond to individual mice. Mean ± SEM of two (B and H) or three (G) pooled experiments is shown, including at least 5 mice per condition. ^∗p < 0.05 and ^∗∗p < 0.01, unpaired Student’s t test comparing WT and LysMΔSHIP-1. #p < 0.05, unpaired Student’s t test comparing the same genotype stimulated or not with β-glucan. In (D) and (F), a pool of two experiments is shown, including between 6 and 16 mice per group as indicated. ^∗∗p < 0.01, log rank test between WT and LysMΔSHIP-1 mice. #p < 0.05, log rank test comparing trained or not with β-glucan (D) or C. albicans (F) within the same genotype.