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. 2018 Nov 9;8:16599. doi: 10.1038/s41598-018-34985-5

Table 3.

Individual immune response of animals from the vaccination study immunized DOA and IM as measured by ELISA (% inhibition), RAPINA and RFFIT (IU/ml).

animal Status B0 (day of capture) B1 (day 28 p.v.) B2 (day 56 p.v.) B3 (day 183 p.i.) Outcome
ELISA RAPINA RFFIT score serostatus ELISA RAPINA RFFIT score serostatus ELISA RAPINA RFFIT score serostatus ELISA RAPINA RFFIT score sero status survival death (days p.i.) FAT/rabies
K22 IM −− + ++−− −1 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K23 IM −− −− −5 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K24 IM −− −− −5 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K26 removed −− −− −5 NEG NO −78 n.t.
K27 IM ++ −− −1 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K29 IM ++−− ++−− −1 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K30 removed ++−− −− −3 NEG NO −78 n.t.
K31 IM −− + −− −3 NEG ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K32 IM ++ + ++−− 3 POS ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K25 removed ++ ++−− 1 POS NO −78 n.t.
K28 IM ++−− + ++ 3 POS ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K33 IM −− + ++ 1 POS ++ + ++ 5 POS ++ + ++ 5 POS ++ + ++ 5 POS YES n.t.
K34 DOA −− −− −5 NEG ++−− −− −3 NEG ++−− −3 NEG NO 12 POS
K35 DOA −− + −− −3 NEG ++ + ++−− 3 POS ++ + ++ 5 POS NO 26 POS
K36 DOA ++−− + −− −1 NEG ++ + ++ 5 POS ++ + ++−− 3 POS NO 15 POS
K37 removed −− −− −5 NEG ++−− ++−− −1 NEG NO −35 n.t
K38 DOA −− ++−− −3 NEG −− ++−− −3 NEG −− ++−− −3 NEG NO 16 POS
K39 DOA −− −− −5 NEG −− −5 NEG −− ++−− −3 NEG NO 13 POS
K40 DOA −− −− −4 NEG −− ++−− −3 NEG −− −− −5 NEG ++ + ++ 5 POS YES n.t.
K41 DOA −− + −− −3 NEG −− ++−− −3 NEG −− ++−− −3 NEG NO 14 POS
K42 DOA −− ++−− −3 NEG −− ++−− −3 NEG −− ++−− −3 NEG NO 18 POS
K43 DOA −− −− −5 NEG −− ++−− −3 NEG −− ++−− −3 NEG ++ ++ 5 POS YES n.t.
K44 removed −− −− −5 NEG NO −66 n.t.
K45 removed −− −− −5 NEG NO −78 n.t.
K46 DOA ++−− + ++−− 1 POS ++ −− −1 NEG ++−− −− −3 NEG ++ + ++ 5 POS YES n.t.

Indeterminate values were considered within a range of variation around the presumed cut-off (0.5 IU/mL +/− 0.25 IU/mL for RFFIT and 40% + 4.41%/− 5.28% for BioPro ELISA) as described previously (Moore et al., 2017). Final rating of the serological status of an individual serum followed a scoring scheme. Every + received a score of +1 and every – a score of −1. The total score was calculated by adding the values; a negative score (<0) was considered sero-negative and a positive score (>0) as sero-positive. Data are stratified according to the serological status at the day of capture (B0). Results of FAT testing are indicated (n.t. – not tested). All animals that survived the observation period of 183 days p.i. were revaccinated and rehomed.