Table 3.
animal | Status | B0 (day of capture) | B1 (day 28 p.v.) | B2 (day 56 p.v.) | B3 (day 183 p.i.) | Outcome | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ELISA | RAPINA | RFFIT | score | serostatus | ELISA | RAPINA | RFFIT | score | serostatus | ELISA | RAPINA | RFFIT | score | serostatus | ELISA | RAPINA | RFFIT | score | sero status | survival | death (days p.i.) | FAT/rabies | ||
K22 | IM | −− | + | ++−− | −1 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K23 | IM | −− | − | −− | −5 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K24 | IM | −− | − | −− | −5 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K26 | removed | −− | − | −− | −5 | NEG | NO | −78 | n.t. | |||||||||||||||
K27 | IM | ++ | − | −− | −1 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K29 | IM | ++−− | − | ++−− | −1 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K30 | removed | ++−− | − | −− | −3 | NEG | NO | −78 | n.t. | |||||||||||||||
K31 | IM | −− | + | −− | −3 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K32 | IM | ++ | + | ++−− | 3 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K25 | removed | ++ | − | ++−− | 1 | POS | NO | −78 | n.t. | |||||||||||||||
K28 | IM | ++−− | + | ++ | 3 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K33 | IM | −− | + | ++ | 1 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | ++ | + | ++ | 5 | POS | YES | n.t. | |
K34 | DOA | −− | − | −− | −5 | NEG | ++−− | − | −− | −3 | NEG | − | − | ++−− | −3 | NEG | NO | 12 | POS | |||||
K35 | DOA | −− | + | −− | −3 | NEG | ++ | + | ++−− | 3 | POS | ++ | + | ++ | 5 | POS | NO | 26 | POS | |||||
K36 | DOA | ++−− | + | −− | −1 | NEG | ++ | + | ++ | 5 | POS | ++ | + | ++−− | 3 | POS | NO | 15 | POS | |||||
K37 | removed | −− | − | −− | −5 | NEG | ++−− | − | ++−− | −1 | NEG | NO | −35 | n.t | ||||||||||
K38 | DOA | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | NO | 16 | POS | |||||
K39 | DOA | −− | − | −− | −5 | NEG | −− | − | − | −5 | NEG | −− | − | ++−− | −3 | NEG | NO | 13 | POS | |||||
K40 | DOA | −− | −− | −4 | NEG | −− | − | ++−− | −3 | NEG | −− | − | −− | −5 | NEG | ++ | + | ++ | 5 | POS | YES | n.t. | ||
K41 | DOA | −− | + | −− | −3 | NEG | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | NO | 14 | POS | |||||
K42 | DOA | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | NO | 18 | POS | |||||
K43 | DOA | −− | − | −− | −5 | NEG | −− | − | ++−− | −3 | NEG | −− | − | ++−− | −3 | NEG | ++ | − | ++ | 5 | POS | YES | n.t. | |
K44 | removed | −− | − | −− | −5 | NEG | NO | −66 | n.t. | |||||||||||||||
K45 | removed | −− | − | −− | −5 | NEG | NO | −78 | n.t. | |||||||||||||||
K46 | DOA | ++−− | + | ++−− | 1 | POS | ++ | − | −− | −1 | NEG | ++−− | − | −− | −3 | NEG | ++ | + | ++ | 5 | POS | YES | n.t. |
Indeterminate values were considered within a range of variation around the presumed cut-off (0.5 IU/mL +/− 0.25 IU/mL for RFFIT and 40% + 4.41%/− 5.28% for BioPro ELISA) as described previously (Moore et al., 2017). Final rating of the serological status of an individual serum followed a scoring scheme. Every + received a score of +1 and every – a score of −1. The total score was calculated by adding the values; a negative score (<0) was considered sero-negative and a positive score (>0) as sero-positive. Data are stratified according to the serological status at the day of capture (B0). Results of FAT testing are indicated (n.t. – not tested). All animals that survived the observation period of 183 days p.i. were revaccinated and rehomed.