Fig. 8.
Defective intermixing is observed in the matrix compartment. a–c DARPP-32 staining in P5 striatal coronal sections reveals that striosomes are still recognizable in Islet1Cre;Ebf1fl/− and Dlx5/6::Cre;Ebf1fl/− (empty arrowheads) (n = 3 for each genotypes and markers). d–i Conversely, in Ebf1 cKOs the matrix compartment is parceled in Enkephalin-rich and Enkephalin-poor (full arrowheads) areas. j–l Intermixing anomalies are prominent in the matrix compartment of both cKOs. In the lateral striatum, iSPN are intermixed both within and outside DARPP-32 + striosomes in controls (j and high magnification), whereas iSPN are intermixed in striosomes (delineated by dotted lines) but not in the matrix of Ebf1 cKOs (high magnifications). m, n Schematic representation of the functions of Ebf1 in dSPN differentiation and in the non-cell autonomous regulation of iSPN migration. n = 3 for each genotype. Scale bars equal 200 μm (a–l) and 30 μm (bottom panels, high magnification). Str striatum