Figure 3.
Expression of DPP4 and host cell proteases in target cell lines and lung tissue. Total cellular RNA was extracted from 293T, Caco-2 and Vero E6 cells, reverse-transcribed into cDNA and quantified for DPP4 and protease transcript numbers by quantitative RT-PCR. cDNA from human lung tissue was also included in the analysis. The numbers of DPP4/protease mRNA copies are shown relative to the housekeeping gene ß-actin (ACTB). Error bars indicate standard deviation (SD). Crosses indicate samples for which no transcripts were detected.