Figure 3.

Effect of local pulmonary HDT with IL10R1-7, IL10R1-14 and ST3-H2A2 peptide inhibitors on the stat3 and Il-10 transcripts and protein levels. (a,c) Lung samples from mice as in Fig. 2 were analyzed by RT-PCR for mRNA transcript expression of stat3 and il10 using lung homogenates based on the expression of 18S. Briefly, the lungs were harvested and samples were collected in Trizol for RNA extraction and qRT-PCR assay was performed to quantify stat3 or il-10 mRNA. (b,d) - Lung homogenates as in Fig. 2 were assayed by ELISA for pSTAT3 or by CBA for IL-10 cytokine. Peptide inhibitor treatment regimens are indicated as: No treatment, IL10R1-7, IL10R1-14 and ST3-H2A2 treatment. Data represent mean ± SEM where *p < 0.05; **p < 0.01; ***p < 0.001 and ns; no statistical significance.