Fig. 9.

PNN degradation facilitates hyperactivity in excitatory neurons. a Representative current-clamp (I = 0) recording traces from an excitatory neuron showing interictal and ictal-like discharges induced by Mg²⁺ removal from ACSF. Expanded trace shows ictal-like activity. b−e Representative voltage-clamp recordings of excitatory neurons from a control (b) and ChABC-treated slice (e) before and after removing Mg²⁺ from ACSF. The expanded traces in (c) and (d) correspsond to before and during interictal-like discharges, respectively, in the recording shown in (b). f−g Latency to Mg²⁺-free-induced (f) interictal activity (control, 17.52 ± 0.81 min, n = 12(6); ChABC-treated, 15.06 ± 1.27 min, n = 8(5); unpaired t test) and (g) ictal-like activity (control, 73.67 ± 6.69 min, n = 12(6); ChABC-treated, 38.83 ± 3.40 min, n = 8(5); ***P < 0.001, Welch's t test). n = cells(mice) in (f) and (g)