Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 9;8:16636. doi: 10.1038/s41598-018-35109-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ROCK2 and STAT3 ChIP-seq analysis of human T cell. Human peripheral blood CD4⁺ T cells were stimulated under TH17-skewing conditions for 48 hours; chromatin was purified and proceeded to ChIP-seq analysis with anti-ROCK2 or anti-STAT3 antibodies. (a) ROCK2 binding is enriched at genomic structures compared to control. Pie chart of ROCK2 active peaks distribution over control. (b) ROCK2 preferentially binds to transcription start sites. Metagene analysis of ROCK2 occupancy on an average gene (left) and Heat map of ChIP-seq reads for ROCK2 occupancy (right). (c) Venn diagram with numbers of genomic sites bound by ROCK2 and STAT3. (d) An integrative genomics viewer (IGV) browser representation of normalized ChIP-seq reads for ROCK2, STAT3 and input at the Irf4 and Bcl6 gene locus. Black bars (Irf4-A and Irf4-B, Bcl6-A) indicate primers used in the ChIP-qPCR analyses in Figs 3, 4.