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. 2018 Nov 9;9:4731. doi: 10.1038/s41467-018-07192-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Imaging of the Drosophila chemosensory system of a FlyClear treated intact adult fly using optimised aspheric ultramicroscope. a–d Light-sheet images of GFP expression in sensory neurons. a Lateral view of olfactory and gustatory receptor neurons in antenna (ANT), maxillary palp (MX) and labellum (LB). The inset shows a higher magnification of the maxillary palp. b Pharynx and the connection of the pharyngeal nerve (PhN) with the ventral cibarial sense organ (vcso), the labral sense organ (lso), and the connection of the labellum with the labial nerve (lbn). The inset shows a higher magnification of the vcso with single neurons. c Dorsal view of antennal receptor neurons, which are connected with the antennal lobe (AL) (dashed box) through the antennal nerve (AN). d Lateral view of the connection described in c. Images in a–c were acquired with a 10x water-immersion objective (Olympus, UMPlanFLN, 0.3 NA, WD = 3.5 mm) with custom-made correction of optics for a refractive index of 1.45 (WD = 3.5 mm after correction). In a, b a post-magnification of 2x was used. Image in d was acquired with a 0.5x post-demagnification in combination with a 25x objective (Olympus, XLPlanN, 1.0 NA, WD = 8 mm). Genotype: a, b dscam-Gal4/CyO; UAS-mCD8::GFP and (c–e) Peb-Gal4 UAS-mCD8::GFP. Scale bars represent 200 µm in a–d and 50 µm in the magnified areas of a, b