Table 1.

Characteristics of the reconstructed carbon assimilation pathway of cassava for pre-MeRecon and MeRecon, including EC numbers, enzymatic reactions, spontaneous reactions, gap reactions, genes, proteins, and metabolites in each sub-metabolism.

Sub-metabolisms	pre-MeRecon								MeRecon
	EC numbers	Reactions				Genes	Proteins	Metabolites	EC numbers	Reactions				Genes	Proteins	Metabolites
		Enzymatic reactionsa	Spontaneous reactionsb	Gap reactionsc	Total					Enzymatic reactionsa	Spontaneous reactionsb	Orphan reactionsd	Total
Calvin cycle	21	23	—	—	23	128	158	35	21	23	—	—	23	128	158	35
Sucose biosynthesis	15	16	—	—	16	64	79	25	15	16	—	—	16	64	79	25
Starch biosynthesis	13	14	—	—	14	82	106	19	13	14	—	—	14	82	106	19
Respiration	36	40	—	—	40	262	325	49	36	40	—	—	40	262	325	49
Amino acid biosynthesis	76	87	2	3	89	250	304	121	78	89	2	1	92	254	308	122
Cell wall biosynthesis	6	6	—	—	6	85	91	15	6	6	—	—	6	85	91	15
Fatty acid biosynthesis	12	35	—	—	35	59	65	49	12	35	—	—	35	59	65	49
Nucleotide biosynthesis	34	51	—	1	51	123	146	67	35	52	—	—	52	125	148	67
Total	196	253	2	4	259	864	1,046	259	199	256	2	1	259	870	1,052	259

^aEnzymatic reactions are catalyzed by enzymes to perform their functions.

^bSpontaneous reactions can perform functions automatically without regulatory enzymes.

^cGap reactions are reactions that require enzymatic proteins for producing essential components, but their encoding genes could not be identified.

^dOrphan reactions are reactions of which the related enzymatic genes have not been yet identified, but have been biochemically characterized.