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. 2018 Nov 9;8:16633. doi: 10.1038/s41598-018-34747-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FGF-2, but not ASC-CMed, inhibits the protein expression of mesenchymal markers. (A) SM22α, and (B) αSMA, by immunoblotting of NHCF-V after stimulation with TGFβ1 or co-stimulation with TGF-β1 and FGF-2, both in FBM and ASC-CMed, for five days. Data were analyzed by One-way ANOVA with Sidak’s multiple comparison test for the groups FBM/TGF-β1 vs. FBM/TGF-β1/FGF-2 and FBM/TGF-β1 vs. ASC-CMed/TGF-β1; p-values for the Sidak’s multiple comparison test are shown in the figure. Values represent mean ± SEM of 3 independent experiments.