Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 9;8:16618. doi: 10.1038/s41598-018-34995-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Generation of kidney organoids through aggregation of mESC-derived nephron progenitor cells and embryonic UB. (A) Schematic of the differentiation protocol of mESCs into kidney organoids. (B) Electrophoresis gel of RT-PCR products presenting kidney lineage cells expressing nephron progenitor markers Cited2, Wt1, Hoxd11, and Six2 after incubation in NPSR medium overnight. (C) Global bright field images of kidney organoids in Trowel culture. Scale bars: 500 μm. (D–F) Whole-mount immunofluorescence analyses of the organoids showing nephron progenitor markers: (D) glomeruli marker - Wt1, (E) proximal tubule marker - LTL, (F) nephron marker - Pax2. Scale bars: 20μm. (G) Confocal image showing three compartments of segmented nephron, including the distal tubule (DT, Pax2 + LTL−), proximal tubule (PT, LTL+) and the glomerulus (G, Wt1+). Dotted box shows single nephron that was used to generate a schematic diagram in (G’). Scale bars: 20 μm.