Fig. 1.

RES attenuates TAC-induced cardiomyocyte apoptosis in the heart. A, Representative images of TUNEL staining the heart (red, left), and quantification of TUNEL-positive nuclei (right, n = 8). Cardiomyoytes were immunostained with α-actinin (green), and nuclei was conterstained with DAPI (blue). B, Representative immunoblotting analyses of Bax, Bcl-2 and cleaved capsase-3 PTEN in the heart after sham operation (left). Quantification of the relative protein levels (right, n = 4). C, Representative immunoblotting analyses of Bax, Bcl-2 and cleaved capsase-3 in the heart after TAC operation with or without RES treatment (left). Quantification of the relative protein levels (right, n = 4). The results are normalized to the GAPDH content. Data are presented as mean ± SEM, and n represents number of animals, *P < 0.05 versus Sham, ^#P < 0.05 versus TAC+RES.