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. 2018 Nov 7;100(3):651–668.e8. doi: 10.1016/j.neuron.2018.08.037

Figure 5.

Figure 5

PD2a1 and PD2b1 Decrease Response to the CS+ after Training

(A) GCaMP3 was expressed in PD2a1 and PD2b1 with R37G11-GAL4. Olfactory responses to Oct and Mch were recorded in vivo from the axonal compartment of PD2a1 and b1 neurons.

(B and C) In naive flies, the calcium increase in PD2a1 and PD2b1 neurons in response to Oct was larger than Mch (average traces from n = 6 flies; t test, p = 0.015; B, average time trace; C, bar chart of response integral).

(D–D”) Odor responses were recorded 3 hr after single-cycle training using Oct as CS+ (n = 19 flies) or after the corresponding unpaired control protocol (n = 20 flies) (Figure S9A).The integral of the odor responses (D’; t test, p = 0.023) and the calculation of the difference between Oct and Mch responses (D”; t test, p = 0.024) revealed a decreased response to the CS+ after the associative protocol.

(E–E”) Odor responses were recorded 3 hr after single-cycle training using Mch as CS+ (n = 22 flies) or after the corresponding unpaired control protocol (n = 21 flies) (Figure S9B). The integral of the odor responses (E’; p = 0.047) and the calculation of the difference between Mch and Oct responses (E”; t test, p = 0.041) revealed a decreased response to the CS+ after the associative protocol.

(F–F”) Odor responses were recorded 24 hr after spaced training using Oct as CS+ (n = 9 flies) or after the corresponding unpaired control protocol (n = 11 flies) (Figure S9C). The integral of the odor responses (F’; t test, p = 0.036) and the calculation of the difference between Oct and Mch responses (F”; t test, p = 0.035) revealed a decreased response to the CS+ after the associative protocol.

(G–G”) Odor responses were recorded 24 hr after spaced training using Mch as CS+ (n = 9 flies) or after the corresponding unpaired control protocol (n = 9 flies) (Figure S9C). The integral of the odor responses (G’; t test, p = 0.047) and the calculation of the difference between Mch and Oct responses (G”; t test, p = 0.010) revealed a decreased response to the CS+ after the associative protocol.

∗p < 0.05. Data are presented as mean ± SEM. Gray bars indicate periods of olfactory stimulation. See also Figure S9.