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. 2018 Nov 7;100(3):651–668.e8. doi: 10.1016/j.neuron.2018.08.037

Figure 8.

Figure 8

PD2a1 and PD2b1 Axons Interdigitate and Interact with DAN Dendrites and MBON Axons

(A) Histogram of light microscopy overlap between a mask of PD2a1 and PD2b1 axons and masks of the dendrites of DANs (along the x axis).

(B and B’) Confocal imaging of double labeling between PD2a1 and PD2b1 axons (labeled with GFP, green) and DAN dendrites (labeled with RFP, magenta). PAM-β’1 dendrites (B) and PAM-β’2m dendrites (B’).

(C) Histogram of light microscopy overlap between a mask of PD2a1 and PD2b1 axons and masks of the dendrites of most MBONs (along the x axis).

(D–D”) Confocal imaging of double labeling between PD2a1 and PD2b1 axons (labeled with GFP, green) and MBONs (labeled with RFP, magenta). Shown are MBON-β’2mp axons (D), MBON-y2ɑ’1 axons (D’), and MBON -ɑ′2 axons (D”).

(E) Visualization of MBON-ɑ’2 axons interdigitating with PD2a1 and PD2b1 axons (black) in the EM volume. Other PD2a1 and PD2b1 neurons are shown in gray. Inset: positions of axo-axonic connections from MBON-ɑ’2 onto PD2a1 and PD2b1 neurons, shown as cyan spheres.

(E’) Summary of ipsilateral MBON-ɑ’2′s axo-axonic connectivity onto PD2a1 and PD2b1 cells. Double labeling images are examples from n = 3 brains. For double labeling, the scale bar represents 5 μm.