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Overexpression of GFP:Repo-manTA3 (GFP-RMTA3), a hyperactive form of Repo-man, causes dephosphorylation of CENP-A Ser7ph in early mitosis (compare panels 4 with 9). DT40 cells were transfected with GFP-RMTA3 then fixed and stained for CENP-A phSer7 (A). The intensity of CENP-A phSer7 at each centromere was measured and quantified (B). Scale bars: 15 μm. Experiment conducted by Thomas William Monteiro Crozier.
