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Gene expression analysis of the anti-chondrogenic paracrine factors in the embryonic hip joint. Expression of aggrecan (a,f,k; cartilage marker), GDF5 (b,g,l; interzone marker), noggin (c,h,m), chordin (d,i,n) and wnt4 (e,j,o) in NR18 gecko (a–e), TK16 turtle (f–j) and HH30 chicken (k–o). Gecko chordin signal was detected in the notochord in the same sections (not shown). pel: pelvis; fe: femur; arrows: the femoral head signal; arrowheads: the hip interzone signal. Scale bars, 300 µm.
