Figure 1.

Virus inoculum, replication at 24 h postinfection, and cell cytotoxicity. Similar levels of Influenza virus replication and cytotoxicity were found in hNECs from young and older individuals. hNEC cultures were infected with an H3N2 strain of influenza virus at an MOI of 10 TCID₅₀/mL at 33°C. Virus titers in inoculum (A) and apical supernatants at 24 h postinfection (B) were measured by calculating the TCID₅₀/mL in MDCK. Cell death was determined by measuring lactate dehydrogenase (LDH) in the basolateral chamber and normalized to mock-infected hNECs from both young and older individuals (C). n = 6 different donors for young and n = 7 donors for older adults.