Figure 3.

hNECs from older subjects express significantly less MHC-I and antigen-processing mRNA and protein. (A, C, D, and F) mRNA was compared in mock and influenza-infected hNECs 24 h postinfection using Nanostring nCounter assay (GX Human Immunology v2 kit) (n = 5 different donors for young and n = 7 donors for older adults). (B, E, and G) Protein in mock and influenza-infected hNECs was analyzed by western blot. Protein bands were quantified using chemiluminesence and normalized to amido black staining. n = 5 different donors for young and n = 5 donors for older adults. *p < .05 and **p < .01 compared with hNECs from young individuals as determined by t-test.