Figure 5.

CTL clearance of airway epithelial cells is dependent upon concentration of antigen presentation. NCI-H522, a lung adenocarcinoma cell line expressing HLA-A*02:01, and A549, a lung carcinoma cell line without HLA-A*02:01 expression, were loaded with a dose–response of Influenza M1 peptide (30.0 ng/mL to 2.0 µg/mL) then co-cultured with M1 peptide-specific HLA-A2+ CTLs at a 4:1 CTL:target cell ratio for 24 h at 37°C. Cell death was measured by lactate dehydrogenase (LDH) levels in media.