Table 6.

Anti-allergic activity of compounds isolated from B. rupestris

Sample	% viability, RBL-2H3a		% inhibition of A23187-induced β-hexosaminidase releaseb
	100 μM	500 μM	10 μM	100 μM	500 μM
β-amyrin acetate (1)	98.3 ± 2.9	c	0.3 ± 0.6	0.7 ± 1.2	c
scopoletin (4)	96.0 ± 4.0	93.7 ± 6.5	3.7 ± 4.7	13.7 ± 8.0	23.0 ± 8.0**d
β-sitosterol-3-O-β-D-glucoside (5)	96.0 ± 1.0	c	5.0 ± 5.6	7.7 ± 3.8	c
dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside (6)	97.3 ± 2.5	e	7.0 ± 10.4	12.7 ± 7.6	e
dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (7)	97.7 ± 2.1	95.3 ± 4.2	3.3 ± 4.2	16.3 ± 5.5*	18.0 ± 8.7*

A mixture of β-sitosterol (2) and stigmasterol (3) was toxic towards RBL-2H3 cells at the concentration of 200 μg/mL (73.7 ± 11.7% viability) and 100 μg/ml (78.7 ± 11.6% viability) and inactive at the concentration of 10 μg/mL (5.0% ± 5.0% inhibition) in A23187-induced degranulation assay

^aThe cytotoxicity of samples to RBL-2H3 was evaluated using MTT viability assay; results are presented as mean ± SD (n = 3)

^bDexamethasone (10 nM) was used as the positive control and inhibited 93.7 ± 1.5%^** of A23187-induced β-hexosaminidase release in RBL-2H3 cells. Results are presented as mean ± SD (n = 3); ^*p < 0.05, ^**p < 0.001 compared with the control value (A23187 only)

^cPrecipitate was formed upon the addition into the medium at the concentration of 500 μM, therefore the result could not be justified

^dScopoletin (500 μM) exerted 30.0 ± 7.1% inhibition of antigen-induced β-hexosaminidase release (mean ± SD, n = 2)

^eDihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside was not tested at the concentration of 500 μM, however, it was nontoxic towards RBL-2H3 cells (96.0 ± 6.9% viability) and inactive in A23187-induced degranulation assay (10.0 ± 4.6% inhibition) at the concentration of 200 μM