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. 2018 Nov 10;13:8. doi: 10.1186/s13008-018-0041-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cellular proliferation is impaired upon over-expression of ALADIN, PGRMC1 or PGRMC2. Equal cell numbers of a wild-type NCI-H295R1-TR, scrambled shRNA and AAAS shRNA (AAAS knock-down) expressing NCI-H295R1-TR cells and of b wild-type NCI-H295R, GFP, GFP-ALADIN, PGRMC2-GFP and GFP-PGRMC1 expressing NCI-H295R cells were seeded 48 h before confluence measurement using live cell imaging on IncuCyte Zoom (Essen BioScience). In case of transient transfection with PGRMC2-GFP and GFP-PGRMC1 the proliferation of fluorescent cells which starts at cell density of about zero was measured. Growth curve analysis and growth constant k (slope of regression curve) calculation was done using multilevel regression technique using R Studio [58, 59]