Fig. 2.

PGRMC1 is restricted to the mitotic spindle and to the mid-body during cytokinesis. a Human adrenocortical NCI-H295R cells at different cell division stages were stained with anti-PGRMC1 (green), anti-Aurora kinase A (AURKA) (red) and DAPI (blue). b Human adrenocortical NCI-H295R cells at meta- and telophase were stained with anti-PGRMC1 (green), anti-α-tubulin (TUBA) (red), anti-β-tubulin (TUB) (red), anti-PGRMC2 (red), anti-Aurora kinase B (AURKB) (red) and DAPI (blue). Scale bars: 5 μm. c Schematic drawing of cellular localization of ALADIN, Aurora kinase A, PGRMC1 and PGRMC2 during mitosis. MT, microtubule