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Improvement of glucose uptake via the activation of IRS-1 and its downstream signaling pathways in C2C12 myotubes by gossypol (GSP). (a) mRNA expression was analyzed by RT-PCR. (b) Total cell lysates were extracted and subjected to Western blot analysis with the designated antibodies. (c) The immunoblotting signals were quantified using densitometer. Data are shown as means ± SEM, ∗ p < 0.05 and ∗∗ p < 0.01, versus no treatment. Rosi: rosiglitazone.
