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Polysiphonia japonica extract (PJE) promotes insulin secretion in zebrafish. Zebrafish were incubated with 10 μg/mL PJE from 3 to 4 days postfertilization. (a) Heart rates of embryos were measured for 3 min. (b) Phase contrast images of zebrafish and fluorescence and confocal microscopy images of the β-cell mass of zebrafish. Scale bar: 200 μm. (c) Relative EGFP fluorescence levels from (b). (d) Number of β-cells per embryo from (b). n = 17–24. ∗ p < 0.05; n.s.: no significance.
