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. 2018 Oct 28;2018:9502451. doi: 10.1155/2018/9502451

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Copyright © 2018 Asmita Banerjee et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Release of immunoactive substances. Release of immunoactive substances in the cell culture supernatant of hAMSCs of reflected and placental amnion was measured after 4 days (5% and 20% oxygen) incubation (a)–(d). Higher oxygen tension (20%) lead to significantly higher secretion of IL-6 (a) and a trend to higher IL-10 release (b) in hAMSCs from placental amnion and had no effect on the release of hepatocyte growth factor (c) and IL1-beta release (d) compared to lower oxygen tension (5%). Cells from placental amnion released more IL-6 (a), IL-10 (b) and hepatocyte growth factor (c) when compared within cultures at same oxygen concentrations (5% and 20% oxygen). n = 6 (biological replicates), mean ± SD. Abbreviations: hAMSCs: human amniotic mesenchymal stromal cells; IL: interleukin.