Fig. 6.

Vav proteins regulate 3D EMT using Zeb2- and E-cadherin-independent mechanisms. a Defects shown by the indicated 4T1 cell derivatives in primary tumorigenesis and specific steps of the metastatic dissemination to the lung [26]. The epithelial and mesenchymal status in 2D and 3D obtained in this work are also included. b Representative example of the morphology of indicated 4T1 cell lines in 2D culture (n = 3 independent experiments). Scale bar, 50 μm. c Representative immunoblot showing the abundance of indicated endogenous proteins in lysates from 4T1 cell line derivatives shown on top. Actin has been used as loading control (n = 3 independent experiments). d Representative example of the morphology of indicated 4T1 cell lines in 3D culture (n = 3 independent experiments). Scale bar, 50 μm