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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2018 May 11;13(3):355–370. doi: 10.1007/s11481-018-9788-3

Fig. 6.

Fig. 6

Beclin1 is independent of oxidative stress induced by Tat with limited contribution of morphine ROS production was assessed by dichlorofluorescein diacetate fluorescence (DCF) over a period of 24 hours following the indicated treatments to C57BL/6J (a) and Becn1+/− (b) glia. Mean DCF relative fluorescence was used as an estimate of ROS and was compared for each treatment. Results are the mean ± SEM from 6 independent experiments. NO production by C57BL/6J (c) and Becn1+/− (d) glia were examined at 0, 1, 6 and 24 hours following the indicated treatments. Nitrite levels were assessed using the Griess reaction with conversion of known concentrations of nitrate to nitrite as standards. Results represent the mean ± SEM of 3 independent experiments. P < 0.05 * vs. Control; # vs. C57BL/6J; $ vs. Tat