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. 2018 Nov 5;11:402. doi: 10.3389/fnmol.2018.00402

Figure 5.

Figure 5

PDGFR-β expression profiles and their spatial relationship with vasculature and collagen in the control and lesioned striatum. (A–D) Triple-labeling for PDGFR-β, GFAP and the endothelial cell marker RECA1 at days 14 (A,B) and 28 (C,D), showing that PDGFR-β expression is present in both vascular profiles and the surrounding extravascular neural tissue in the lesion core. Note the more-prominent enrichment of PDGFR-β expression in extravascular areas at 28 days. The broken lines indicate the border between the peri-lesional area and the lesion core, in which GFAP immunoreactivity is absent. (B,D) Higher magnification images of the boxed areas in (A,C), respectively. (E–G) Double-labeling for PDGFR-β and NG2, showing that the extravascular network, mainly composed of PDGFR-β-positive processes, is devoid of NG2 expression. (H) Quantitative temporal analysis showing that the extravascular area occupied by PDGFR-β expression in the lesion core increases significantly until 28 days. (I) Quantitative temporal analysis showing the progressive decrease in the maximum horizontal distance of the lesion core over time. The data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001 vs. saline-treated controls or the day 3 data, respectively. (J–O) Triple-labeling for PDGFR-β, RECA-1 and collagen IV in the control (J,K) and lesioned striatum at days 7 (L,M) and 28 (N,O) post-lesion. PDGFR-β-positive vessels in the controls show very weak immunoreactivity for collagen IV, but they reveal intense collagen expression at 7 days post-lesion. At 28 days, a close relationship between PDGFR-β and collagen IV can be observed in the lesion core, where both are observed in vascular and extravascular areas. (P) Histogram of the intensity profiles of PDGFR-β-positive and collagen IV-positive signals along the indicated area (white arrows in N,O). Note that the two signals share overlapping spatial profiles within vascular (RECA-1-positive) and extravascular areas. Cell nuclei are stained with DAPI. Scale bars = 100 μm for (A,C); 20 μm for (B,D,E–G,J–O).