Figure 9.

Characterization of extravascular PDGFR-β-positive cells in the lesioned striatum at days 14 and 28 after 3-NP injection. (A–H) Triple labeling for PDGFR-β, GFAP and nestin at day 14 (A,C–E) and 28 (B,F–H) post-lesion showing that the distribution of PDGFR-β overlaps well with that of nestin. Note that PDGFR-β-positive processes show finer intertwined profiles, while nestin staining reveals distinct fibrous structures. (C–H) Higher magnification images of the boxed areas in (A,B), respectively. (I–N) Double labeling for nestin and either laminin (I–K) or fibronectin (L–N), showing that nestin is colocalized with two stromal cell markers. (O,Q–T) Triple labeling for PDGFR-β, nestin and collagen IV showing that expression of the three proteins generally overlap in the lesion core. The boxed area in (O) is enlarged in (Q–T). (P) Histogram of the intensity profiles of PDGFR-β, nestin and collagen IV along the indicated area (white arrows in Q–S) showing that the three signals share overlapping profiles. Cell nuclei are stained with DAPI. Scale bars = 100 μm for (A,B,O); 20 μm for (I–N„Q–T); 10 μm for (C–H).