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. 2018 Nov 5;12:392. doi: 10.3389/fncel.2018.00392

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Copyright © 2018 Liu, Liu, Wang, Wang, Dong, Wu, Qu, Liu, Liu, Fan and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of transient receptor potential vanilloid 4 (TRPV4) was increased in the cuprizone (CPZ)-treated mice. Immunofluorescence staining of 2′,3′-cyclic nucleotide 3′ phosphodiesterase antibody (CNP) (A,B) and TRPV4 (C,D) in the corpus callosum of the control (Ctrl) group and the CPZ group. Scale bar = 20 μm. (E) Integrated optical density (IOD) analysis of CNP- and TRPV4-positive cells. n = 4 per group. The data are shown as the mean ± SEM. **P < 0.01, ***P < 0.001 vs. the Ctrl group.