Figure 2.

Red extract is as effective as ASA in inhibiting the trigeminal infiltration of Iba1+ macrophages in CFA-injected rats. (A–F) Representative micrographs of Iba1 staining (green) in the ipsilateral trigeminal ganglia (TG) of CFA-injected animals drinking water (A,D), Yellow (B,E) or Red (C,F) extracts, treated with saline solution (A–C) or 50 mg/kg ASA (D–F). Nuclei have been counterstained with the Hoechst33258 dye. Scale bars: 50 μm. The specificity of the anti-Iba1 primary antibody is shown in Supplementary Figure S1. (G) Evaluation of the number of Iba1+ cells/area in the ipsilateral TG of CFA-injected animals drinking water, Yellow or Red extracts and treated with saline (light colored-bars) or 50 mg/kg ASA (dark colored-bars). Animals have been sacrificed at 72 h post CFA injection (see protocol in Figure 1 and text for details). N = 4 (Water), 3 (Yellow) and 5 (Red) rats treated with saline, and three (Water, Yellow and Red) rats treated with ASA. **p < 0.01 and ***p < 0.001 with respect to purple corn supplement, two-way ANOVA followed by Bonferroni test; ^#p < 0.05 with respect to corresponding Saline values, Students’ t-test.