Fig. 5.
A: gastrocnemius protein expression via Western blot of mitochondrial markers (SMHC/PGC-1α and TFAM/CS expression measured on same blots with same loading control, due to size similarities). TFAM, mitochondrial transcription factor A; CS, citrate cynthase; HSP60, heat shock protein 60; MTCO1, mitrochondrial complex 1; HSP60, heat shock protein 60; HLS, hindlimb suspension; Ex, exercise. B: Western blot analysis of soleus muscle mitochondrial markers. AU, arbitrary units. Statistical significance (P < 0.05) between groups indicated by the corresponding group letter. C: Gene expression analysis by qPCR of mitochondrial markers. (groups: n = 6, P < 0.05, means ± SE).