Table 1.
Exercise protocol
| Monday | Tuesday | Wednesday | Thursday | Friday | |
|---|---|---|---|---|---|
| Week 1 | |||||
| Warm-up | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | |
| Work rate | off | 7 m/min | 8 m/min | 9 m/min | 10 m/min |
| Rest | 3 min off/100m | 3 min off/100 m | 3 min off/100 m | 3 min off/100 m | |
| Distance | 300 m | 300 m | 300 m | 300 m | |
| Week 2 | Monday | Tuesday-Sprints | Wednesday | Thursday-Sprints | Friday |
| Warmup | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m |
| Work Rate | 11 m/min. | 15 m/min for 10 m | 12 m/min | 17 m/min for 15 m | 13 m/min |
| Walk Rate | 7 m/min for 10 m | 7 m/min for 15 m | |||
| No. of Sprints | 8 | 10 | |||
| Rest | 3 min off/100 m | 5 min after 5 sprints | 3 min off/100 m | 5 min after 5 sprints | 3 min off/100 m |
| Distance | 350 m | 160 m | 375 m | 300 m | 400 m |
| Week 3 | Monday | Tuesday-Sprints | Wednesday | Thursday-Sprints | Friday |
| Warmup | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m | 7 m/min for 50 m |
| Work Rate | 13.5 m/min | 18 m/min for 15 m | 14 m/min | 20 m/min for 20 m | 14.5 m/min |
| Walk Rate | 7 m/min for 15 m | 5 × −7 m/20 m; 6 × 7 m/10 m | |||
| No. of Sprints | 11 | 11 | |||
| Rest | 3 min off/100 m | 5 min after 5 sprints | 3 min off/100 m | 5 min after 5 sprints | 3 min off/100 m |
| Distance, m | 450 | 330 | 500 | 380 | 550 |
Mouse concurrent treadmill exercise protocol consisting of a week 1 acclimation, followed by progressive training week 2 and week 3. Three days per week (M, W, F) consisted of endurance-style exercise, whereas two days per week (T, TH) consisted of higher-intensity interval sprint style of exercise.