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. 2018 Jul 5;125(4):999–1010. doi: 10.1152/japplphysiol.00137.2018

Table 2.

qPCR primers

Gene Forward Nucleotide Sequence Reverse Nucleotide Sequence
Myh7 5′-ATCAAATCATCCAAGCCAACCC 5′-GAGGAGTTGTCATTCCGAACTG
Myh4 5′-TTGTGGTGGATGCTAAGGAGTC 5′-GTACTTGGGAGGGTTCATGGAG
CS 5′-CACTGTTGAGATGGACACACTG 5′-TCCTGAAGTCTGCATCATGACT
SOD-1 5′-GAACCAGTTGTGTTGTCAGGAC 5′-GCCTTGTGTATTGTCCCCATAC
SOD-2 5′-GGTCACAGTTTCACAGTACACC 5′-TCACAGCCTTGAGTTACAGAGT
TFAM 5′-GAGAGCTACACTGGGAAACCACA 5′-CATCAAGGACATCTGAGGAAAA
PGC-1a 5′-CTTTCTGGGTGGATTGAAGTGG 5′-CTCAAATATGTTCGCAGGCTCA
Rn18S 5′-cacggacaggattgacagattg 5′-gacaaatcgctccaccaactaa
β-actin 5′-ccctgaagtaccccattgaaca 5′-cacacgcagctcattgtagaag

Nucleotide sequences of all examined genes. Myh, myosin heavy chain; CS, citrate cynthase; SOD, superoxide dismutase.