Figure 4.

(A) Scatter plot showing the correlation between the lncRNA and the proximal (<100 kb) protein coding genes. Only proximal coding genes exhibiting differential gene expression (FDR value below 5% and a fold change threshold of ±1.5) are displayed. Correlation calculated using Spearman correlation. (B) Gene ontology analysis of the differentially expressed proximal coding genes generated with IPA (QIAGEN). Numbers in the bars indicate the amount of genes included in the pathways. (C) Scatter plot showing the fold change (log₂) of RNA-Seq compared to the fold change (log₂) of GRO-Seq of the differentially expressed lncRNAs. Transcripts exhibiting low expression (RPKM < 0.5 in <2 samples) were removed from the analysis. Correlation calculated using Spearman correlation.