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. 2018 Nov 5;11:406. doi: 10.3389/fnmol.2018.00406

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Copyright © 2018 Fruhmann, Marchal, Vignaud, Verduyckt, Talarek, De Virgilio, Winderickx and Cullin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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αAβ₄₂ instigated lipid droplet biogenesis. (A) Nile Red stainings (top panel), a marker for lipid droplets, of wild-type and the bro1Δ cells expressing αAβ₄₂wt, αAβ₄₂G37C, or αAβ₄₂L34T or αGFP after 2, 4, or 6 h growth on galactose-containing medium. The bottom panel (yellow) shows magnifications and deconvolved parts of the top-panel pictures at time point of 6 h. Note that due to deconvolution the picture intensities are enhanced. Scale bars represent 2 μm. (B) DHE staining of cells deleted for BRO1 transformed with constructs allowing for expression of αAβ₄₂wt, αAβ₄₂L34T, αAβ₄₂G37C, or αGFP after 4 or 24 h growth on galactose-containing medium. Error bars represent standard deviations of at least four independent transformants.