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. 2018 Nov 5;12:400. doi: 10.3389/fncel.2018.00400

Figure 1.

Figure 1

P7C3 inhibited lipopolysaccharide (LPS)-induced production of pro-inflammatory factors in BV2 cells. (A) BV2 cells were pretreated with P7C3 (0.1, 1, or 10 μM) for 2 h and then exposed to LPS (100 ng/mL) for 24 h. After treatment, the protein levels of iNOS, COX-2 and GAPDH were measured using immunoblot analysis. The quantification of the intensity of iNOS and COX-2 relative to GAPDH was shown in the two panels below. (B) BV2 cells were pretreated with P7C3 (0.1, 1, or 10 μM) for 2 h and then exposed to LPS (100 ng/mL) for 24 h. After culture, the cultured media were collected to measure the levels of nitrite using the Griess reagent, while the levels of PEG-2, IL-6 and TNFα were detected using ELISA assays. The values were presented as the means ± SEM from three independent experiments. Kruskal–Wallis test followed by the Iman-Conover method for multiple comparison between groups was performed, *P < 0.05, **P < 0.01 vs. the group treated with LPS alone.