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. 2018 Nov 5;12:400. doi: 10.3389/fncel.2018.00400

Figure 4.

Figure 4

P7C3 suppressed LPS-induced degradation of inhibitory κB alpha (IκBα) by inhibiting IκB kinase (IKK) activation. (A) BV2 cells were treated with P7C3 (10 μM) for 2 h followed by the treatment with LPS (100 ng/mL) for 10 min. The IκBα and α-Tubulin protein levels were detected using immunoblot analysis. The bottom panel showed the band intensity of IκBα to α-Tubulin. The values were presented as the means ± SEM from three independent experiments. Mann and Whitney for comparison between groups, *P < 0.05. (B) BV2 cells were pretreated with P7C3 for 2 h and then exposed to cyclocheximide (CHX; 200 μg/mL) for the indicated time. The IκBα and α-Tubulin protein levels were detected using immunoblot analysis. The bottom panel showed the band intensity of IκBα to α-Tubulin. The values were presented as the means ± SEM from three independent experiments. Kruskal–Wallis test followed by the Iman-Conover method for multiple comparison between groups was performed, *P < 0.05. (C) BV2 cells were treated as (A). The p-IKK, IKK and α-Tubulin protein levels were detected using immunoblot analysis. The bottom panel showed the band intensity of p-IKK to IKK. The values were presented as the means ± SEM from three independent experiments. Mann and Whitney for comparison between groups, *P < 0.05.